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Each biological replicate was assayed in triplicate.
Each biological replicate was assayed independently.
A single qRTPCR run contained four biological replicates, and each biological replicate was assayed in triplicate.
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Two separate biological replicates were assayed for the clock shift assay and the assays using light to deprive flies of sleep; four separate replicates were performed for the mechanical sleep deprivation assays.
Thereafter, 3 – 4 biological replicates were assayed using one technical replicate.
Two biological replicates were assayed for each ChIP-qPCR experiment.
Three independent biological replicates were assayed for each condition (i.e. vehicle control or COX-2 inhibitor).
A total of three biological replicates were assayed (3 control, 3 treated) where each pooled 2 g sample represented a single biological replicate.
In order to evaluate the reproducibility of our experimental samples and procedures, two independent biological and technical replicates were assayed for each gestational age and each allele resulting in 36 (n = 3 × 3 × 2 × 2) samples for sequencing.
For real-time quantitative RT-PCR experiments, we repeated the same biological design than what previously described for micro-array assays; a fourth biological replicate was used for control larvae (injected with PBS) in the HdIV assay.
Two or three biological replicates, with three technical replicates from each biological replicate were performed for each assayed gene.
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