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All the potentially affected biological processes were ranked according to the overall P value, with a cut-off of <0.01.
Over-represented biological processes were ranked according to their position in the GO hierarchical scheme [15] (see Methods).
Biological processes were ranked according to their significance of enrichment, and the validation mode measure of significance was used to identify those of greatest enrichment.
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Biological processes are ranked based on increasing adjusted p-values (from the most to the least significant biological process).
Then each biological process was ranked according to the number of involved target genes.
The top 3 biological processes were metabolic processes, regulation of biological processes, and response to stimulus.
Overrepresented biological processes were glycolysis and several other metabolic processes.
GOrilla uses hypergeometric statistics to determine whether particular biological processes are enriched within any ranked input set of genes.
Functional annotation of the 418 target genes of differential miRNA revealed that high ranked biological processes are transcription regulation, protein modification and photosynthesis.
Numerous biological processes are regulated by enzymes.
Nutrients losses in biological processes are inevitable.
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