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Molecular functions as well as biological processes were compared for genes targeted by both conserved and non-conserved miRNAs to detect any bias in biological function.
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Based on Gene Ontology analysis of the target genes of unedited and edited let-7a, the genes involved in "biological process" were compared between two groups.
Then, the fold change of the genes associated to each biological process was compared to the overall distribution of fold changes.
The retention rates of genes differentially expressed in each biological process are compared to the genome wide values for all 39,642 protein coding genes.
Their GO slim assignments for Biological Processes were statistically compared in Blast2Go to test for enrichment of particular GO terms using Fisher's Exact Test.
The same biological processes were enriched compared with either human plasma proteome or total human genome calculated proteome, suggesting an active enrichment of plasma proteins in the lung rather than passive capillary leak.
In general, affected dose-specific biological processes were more frequent compared to the affected processes observed at all absorbed dose levels.
The following biological processes were over-represented compared with all gonad-expressed genes: cell cycle (P < 0.005); glucose metabolism (P < 0.007); and post-transcriptional gene regulation (P < 0.04).
In the gln1.4 mutant the same biological processes were altered, however compared to the gln1.3 mutant, there were much fewer proteins present in lower (10 proteins) or higher amounts (4 proteins).
When down-regulated GO biological process terms were compared, 2,4DNT and 2,6DNT were more similar than other compounds (Table 7).
The enriched genes for each biological process group were compared between the Quarter Horse and reference Thoroughbred using the Fisher's exact test for a 2 × 2 contingency table.
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