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Unique to BMB is the combination of powerful molecular biological methodologies (e.g. cloning, gene splicing and gene expression), with biochemical and biophysical assays, as well as structural biology strategies (e.g. X-ray crystallography, 2-D NMR, cryo-electron microscopy) for dissecting structure and function of macromolecules.
This view is re-enforced by an ignorance of biological methodologies (Aroua et al. 2005) and biological teachings that equate biology with physics and do not consider the historical dimension of biology (Aroua et al. 2007).
Furthermore, biological methodologies of different tissue staining methods and inductively coupled plasma (ICP) yielded consistent results, proving that the obtained in vivo results occurred because of targeted anti-CEA SPIONPs.
Many well-established molecular and biological methodologies including microarray, cloning, northern blotting, quantitative real-time-PCR (qRT-PCR), in situ hybridization (ISH), and next generation sequencing (NGS) are now being successfully utilized for miRNA research [12], [13], [14], [15], [16], [17].
Importantly, there are some discrepancies that highlight several limitations of the bioinformatics and biological methodologies.
It is now possible to uncover novel molecular tumour markers not elucidated by the traditional and molecular biological methodologies mentioned above.
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Since developments in synthetic methodology tend to be incorporated into new library development, and improvements in analytical and biological methodology are applied to screening systems, chemical genetics research often encapsulates the best of both chemistry and biology.
In the same way, the first kind of philosophy of biology I have described the use of biological examples to work through general issues in the philosophy of science sometimes feeds back into biology itself through specific recommendations for improving biological methodology.
However, the molecular biological methodology applied provides a deepness that under financial considerations does not allow large-scale studies.
"The collaboration of Professor Joonil Seog, who is an expert on biological assay methodologies and characterization, has been crucial in this respect," Oehrlein notes.
The application of biological imaging methodologies is essentially non-invasive, and thus biopsies before and during the treatment do not need to be taken, leaving the tumour microenvironment undisturbed.
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