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Recently, light has been used as a trigger to control the biological function of small molecules, oligonucleotides, and proteins involved as parts in gene circuits.
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In this review, we give a brief introduction to the discovery of RNAi, describe its molecular mechanisms and discuss the biological functions of small RNAs in insects and the development of new technologies for insect pest control.
Although in silico target predictions have been shown a good tool to putatively annotate the small RNA functions [ 29], the experimental validation of the exact biological functions of small RNAs in plant cells is necessary.
The molecular interaction and, hence, the biological function of a small molecule is related to its three-dimensional structure when interacting with the protein.
The biological function of only a small fraction of these miRNAs have been reported in detail and point to their involvement in a variety of developmental and physiological processes.
As a validation strategy for potential biological function of these predicted microRNAs, we searched for small RNAs that could be associated with RISC by immunoprecipitation (IP) with anti-Ago2.
We focus here on the impact of using RNAi to probe the biological function of proteins for target identification and selection for discovering small molecule drugs.
In the present study, we have explored the biological function of MELK using MELK-T1, a novel and selective small-molecule inhibitor.
To study the biological function of miRNAs, and to achieve sustained or conditional gene silencing with siRNAs, systems that allow controlled expression of these small RNAs are desirable.
"It regulates every biological function of your body".
That is the biological function of hunger -- to remind animals to eat (because otherwise "normies" might forget... for serious).
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