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The expression profiles and biological function of selected miRNAs and mRNAs were analysed in more detail.
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These preliminary data would allow us to prioritize further analyses with an emphasis on understanding of the biological functions of selected genes.
Combining this method with the GO database, biological pathways, disease databases, and relevant literature, we will conduct a further assessment of the relevance of the biological functions of selected informative genes to the mechanisms of disease [ 42].
We assessed the potential biological functions of select miRNAs by identifying putative miRNA targets using TargetScan and miRDB for miR-513a-5p miR-513a-5p miR-513a-5piR-923.
Furthermore, the biological function of these selected hub proteins in ovarian cancer is currently unknown.
The open source database for annotation, visualization and integrated discovery (DAVID version 6.7) [ 28, 29] was employed to assess biological function of the selected genes.
Based on stringent statistical criterion (FDR<0.0005) and/or biological function of interest, we validated selected genes using real-time PCR.
On the basis of the detected selection signatures in the chicken data analysis, further bioinformatics analyses were carried out to reveal the potential biological function of genes located in putatively selected regions.
To explore the biological functions of the genes selected to form the prognostic signature, Ingenuity Pathway Analysis software was used (http://www.ingenuity.com).com
Current analysis is aimed at understanding the distribution and probable biological functions of the select serine proteases encoded in representative prokaryotic organisms.
We have shown previously in a selected set of mutant proteins that chemical chaperones may restore biological function of CBS mutants [ Singh et al., 2007].
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