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Hence, the total set of genes that were transcribed during the biological function of interest can be determined.
The highly parallelized nature with which such libraries can be generated also has the potential to expand directed protein evolution from single protein targets to protein networks whose concerted activities are required for the biological function of interest.
Based on stringent statistical criterion (FDR<0.0005) and/or biological function of interest, we validated selected genes using real-time PCR.
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Eleven genes were selected based on fold-change or biological functions of interest (Table 1).
Our approach should be of interest to a spectrum of biologists: it can be used to sift through large amounts of experimental data, and will help the experimentalist to identify specific genes or biological functions of interest.
Alternatively, a user can specify Genome Properties [ 26] or Gene Ontology (GO) identifiers [ 27] representing entire pathways or broader biological functions of interest, and the HMMs for different protein families attributed to that function are then retrieved automatically as queries.
Currently, most tools used for retrieving bioscience literature are based on keyword searches, although such approaches have limitations: firstly, it can be difficult for a researcher to find a set of keywords that exactly specify the biological functions she or he may be interested in; secondly, the ranking of results is usually not based on relevance to the biological functions of interest.
Modifier: this refers to any drug or drug-like compound or laboratory method that can modulate the biological function of a molecule of interest so to interfere with the efficacy of a therapeutic agent.
Therefore, the overrepresentation of a certain oligonucleotide only in a restricted portion of the BLSOM (and thus of the genomic sequences) is thought to provide useful information for understating the biological significance of the respective sequence, especially when a biological function of the oligonucleotide of interest is known.
This indicates that anti-CRP antibodies might have biological functions of pathogenetic interest in SLE.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com