In descending order, the DNA molecular weight (Mark Shenzhen, Yishengtang Biological Enterprises Ltd). of these receptors are 2,000 bp/1,600 bp/1,200 bp/800 bp/600 bp/400 bp/200 bp.
ELISA diagnostic kits for HBV serologic markers, anti-HAV, anti-HCV, and anti-HEV IgG were all purchased from Beijing Wantai Biological Pharmacy Enterprise Co., Ltd.
The levels of IgM or IgG antibody against HEV were determined by commercial enzyme-linked immunosorbant assays (ELISA) (Beijing Wantai Biological Pharmacy Enterprise Co., Ltd., Beijing, China) according to the manufacturer's instructions.
HIV status test was carried out using an enzyme-linked immunosorbent assay (ELISA, Wantai Biological Pharmacy Enterprise Co., Ltd, Beijing, China) and confirmed by another ELISA kit (Shanghai Kehua Bio-engineering Co., Ltd., Shanghai, China).
All specimens were also tested for IgM antibody against hepatitis A virus (HAV-IgM), IgM antibody against hepatitis B core protein (HBc-IgM), hepatitis B virus surface antigen (HBsAg), and antibody against hepatitis C virus (HCV-Ab) using commercial ELISA kits (Beijing Wantai Biological Pharmacy Enterprise Co., Ltd., Beijing, China).
For diagnosis, an enzyme-linked immunosorbent assay for HEV IgG and IgM (Beijing Wantai Biological Pharmacy Enterprise CO., LTD).
Anti-HIV IgG antibody and HBV surface antigen (HBsAg) were tested using an ELISA technique (Wantai Biological Pharmacy Enterprise Co., Beijing, China).
HEV IgM and IgG were detected by using the PE2 HEV-IgM and IgG ELISA (Wantai Biological Pharmacy Enterprise Co., Ltd., Beijing, People's Republic of China).
Additional HEV IgG- and IgM-specific ELISA tests (Beijing Wantai Biological Pharmacy Enterprise), retrospectively done on a serum sample from day 8 of illness confirmed positive results.
Subjects with a positive result in the first test were retested with another ELISA reagent (Beijing Wantai Biological Pharmacy Enterprise Co., Ltd., China).
