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Therefore, biological duplicates were pooled together for the rest of the study.
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The averages of biological duplicates were compared.
Biological duplicates were performed.
Data from independent biological duplicates were averaged.
Two biological duplicates were performed for each sample.
For Fig 1B, 221 Lgr5+ cells from biological duplicates were quantified.
Biological duplicates were generated for each experimental sample.
Two biological duplicates were set up, including four technological repeats in each duplicate.
In brief, 1 μg total RNA from 5-Aza-CdR treated and untreated LNCaP and PrEC biological duplicates was reverse transcribed with pools of miRNA specific RT-primers, and the resultant cDNA was subjected to real-time reverse transcription quantitative (qRT -PCR using specific forward primers and TaqRT -PCRusingcent probespecificarray forward
Independent biological duplicates are shown.
For qPCR analysis, biological triplicates were pooled and analyses performed in technical duplicate.
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