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Experiments with biological data were performed to obtain realistic values of intensities λ and μ of duplications and losses.
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ANOVA for clinical and biological data was performed to test the differences among groups, and the Bonferroni post hoc test for multiple comparisons was further performed.
This classification was a main aim of the MENA PWID systematic review, wherein a comprehensive analysis of available HIV biological data was performed using rigorous methodology to characterize HIV epidemic state at country-level [ 2].
Fitting of the biological data was performed through minimization of a predefined fitness function in combination with a genetic algorithm provided by the R package GA [ 60], and a parallelized implementation of the model controlled via RNetLogo.
A biological interpretation of the data was performed using two data-mining programs to identify the main biological functions and the main metabolic pathway disorders revealed by the expression profile in EPO-d muscles.
A biological interpretation of the data was performed using two types of analysis to identify the main biological functions and the main metabolic pathway disorders revealed by the expression profile of the PSSM muscles.
The biological interpretation of expressional data was performed using Ingenuity Pathway Analysis 7.0 (IPA, Ingenuity Systems Inc., Redwood City, CA).
The biological interpretation of expressional data was performed using Ingenuity Pathway Analysis 7.0 (IPA, Ingenuity Systems Inc., Redwood City, CA) and Gene Ontology (GO, http://www.geneontology.org/).org/
The replication of qRT-PCR data was performed in biological replicates from n = 3 or 4 animals and n = 2 technical replicates per animal sample (for a total of six or eight data points).
The analysis of SNP-array data was performed in biological triplicates.
All the assays were carried out in technical triplicates with 2 biological replicates and statistical analysis of data was performed using single-factor ANOVA followed by Tukey's post hoc analysis.
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