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For all developed equations presented above, biological consistency with expectation, parameter significance, sign, and magnitude as well as degree of multicollinearity were evaluated.
Several validation methods were developed, but two methods have been commonly used: comparison of model outputs with observed data and evaluation of the biological consistency of the predictions.
For the diameter-increment models, only the independent variables of the pioneer group (Group 5) were not significant (p > 0.05), but they were nevertheless included in the model since the signs of the coefficients exhibited biological consistency.
Biological consistency was explored by comparing two independent studies of peripheral white-blood cell derived samples.
This allows the reader to consider our preliminary results in terms of their statistical and biological consistency given the multiple testing issues.
Overall, the network analysis demonstrated biological consistency between the gene panels.
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The polished apertures were coated with 10 nm of SiO2 through atomic layer deposition (Oxford Instruments plc, FlexAL) to ensure biological compatibility, consistency with other experiments performed on glass coverslips, and greater consistency in supported lipid bilayer formation.
Figure 2, Figure 3 and Figure S4 contain a subset of significantly enriched or depleted GOSlim terms based on their biological meaning, consistency of enrichment or depletion in gene lists determined with different FDR-values, and hypergeometric p-value.
In this exploratory study, we did not want to overlook possible relationships and instead focused on those markers that demonstrated both biological plausibility and consistency in their associations with CT parameters.
Additionally, significant associations were examined for biological plausibility and consistency with other literatures, as discussed later.
All the links in the evidentiary chain of causality – temporality, strength, dose response, specificity, consistency, biological plausibility and experimental confirmation – are in place.
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