An exhaustive biological check-up was conducted to rule out possible organic causes (i.e., immunological, infectious, metabolic, iatrogenic and toxic) [ 14].
The standardized biological check-up performed at HEC included a neuropsychological assessment, screening for syphilis, serum electrolytes determination and serum assays of vitamin B12, vitamin B9, albumin, pre-albumin and Thyroid Stimulating Hormone (TSH).
They were submitted to a clinical examination every 6 months, with growth monitoring, to a biological check-up every year (complete blood count, serum iron and folate, serum vitamin levels, albuminaemia, SGOT/SGPT) and to an x-ray absorptiometry every 2 years.
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Protein identification was performed using a confidence threshold of 95% (Proteinpilot Unused score ≥1.31) with MMTS selected as cysteine modification, and with the search option 'emphasis on biological modifications' checked.
The Paragon algorithm was used with SILAC (Lys + 6, Arg + 10) selected as the Sample Type, iodacetamide as cysteine alkylation, with the search option "biological modifications" checked, and trypsin as the selected enzyme.
Dose adjustments and the addition or withdrawal of concomitant drug therapy can be occasions for biological status checks with laboratory assessments and vital signs, in addition to assessing drug-specific adverse events.
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In today's oceans, iron is in such short supply that it is a limiting nutrient — an essential element whose scarcity holds biological productivity in check.
Microbial assay to check biological contamination confirmed the presence of microbes in filed water as well as in fouled membrane samples.
