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The method has two steps; the first is the imaging of gold nanoparticles that labels targets of interest inside biological cells using a lock-in technique that enables the separation of the signal from the wide spread spectral noise.
We already manipulated microbeads and biological cells using a two-fingered microhand as a contact manipulation method in previous studies [2, 9].
Optical injection is a promising technique for transiently perforating the membrane of living biological cells, using a tightly focused laser beam to allow impermeable molecules, such as drugs, dyes or nucleic acids (including plasmid DNA), to pass into the cytoplasm.
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Images of individual biological cells using mechanical contrast is of great interest as it can provide complementary information to that obtained with conventional optical microscopy.
Particles and biological cells used in this study had positive acoustic contrast factors, which moved them toward the pressure node as a result of the acoustic radiation effect.
The calcein fluorescence level can be measured by a fluorometer, often a microplate reader (MPR), assessing the fluorescence as a whole (cultures isolated from biological samples) [ 19] or cell by cell using a flow cytometer [ 20].
"Methods and products for delivering biological molecules to cells using multicomponent nanostructures", 2008.
Searson P "Methods and products for delivering biological molecules to cells using multicomponent nanostructures", 2008.
In order to appreciate whether anti-ricin scFv could function as an effective therapeutic against ricin intoxication, 43RCA was tested for its ability to neutralize ricin biological activity using a cell-free translation assay.
In a screen for biological function using a cell model of Parkinson's disease,[ 7] iotrochotazine A had specific effects on the early endosome and lysosome markers, while not showing any cytotoxicity in a number of other mammalian, parasite, and bacterial cell-based assays.
Controlling the microenvironment of injected myogenic cells using biological scaffolds enhance muscle regeneration (Borselli et al. 2011).
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