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All of the stress treatments had three biological and temporal replicates.
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All quantitative PCR experiments were repeated with three biological and three technical replicates per biological replicate.
There were three biological and technical replicates respectively.
Three biological and technical replicates were done for each sample.
Biological and technical replicates were done in triplicate.
Three biological and technical replicates were analyzed.
Each treatment was replicated 27 times, comprising nine spatial replicates sampled over three temporal replicates, with the second and third temporal groups defoliated approximately 3 and 7 days after the first temporal group, respectively (see Figure S1 for exact dates).
For zm genotypes: n=2 biological replicates and 3 technical replicates; for st51 genotypes: n=5 biological and 3 technical replicates.
Two biological and two technical replicates were conducted per sample.
Within each temporal replicate, 8 individually potted biological replicates of each variety were examined for each treatment except for B. nigra and B. juncea varieties, which suffered from variable germination.
Twenty-seven biological replicates (three temporal and nine spatial) for each treatment were used, and three technical RT-qPCR replicates were analysed for each biological replicate.
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