Sentence examples for biological and technical variability from inspiring English sources
In this study, we aimed to identify biological and technical variability in RT-qPCR TaqMan® assays performed with FFPE-RNA from lymph nodes of classical Hodgkin lymphoma samples.
While considering the design of a microarray experiment, the sources of biological and technical variability must be considered as they affect the ability to detect true gene expression changes.
We showed that total variability in RT-qPCR from FFPE-RNA is not higher than that reported for fresh complex tissues, and identified gene-, and expression level-sources of biological and technical variability, which can allow better strategies for designing RT-qPCR assays from highly degraded and inhibited samples.
From this value it was impossible to discriminate between biological and technical variability.
However, microarray studies are subject to potential variations including biological and technical variability.
Replicates should be sufficient to allow for both biological and technical variability.
At the scale of a single cell, biological variability (the object of interest) and technical variability (a nuisance factor) are often of the same magnitude, making it difficult to distinguish between the two.
The objective of the study was to characterize the biological and technical components of variability associated with longitudinal measurements of FEV1 and carbon monoxide diffusing capacity (Dlco).
Minor points "There are no error bars to indicate biological or technical variability in Figure 3 or 4. " Error bars have been added to illustrate technical variability in Figure 3 and Figure 4, respectively.
Minor points There are no error bars to indicate biological or technical variability in Figure 3 or 4. The workflow of Figure 1 does not indicate that the right-hand-side pathway involved only PBMC, not whole blood.
It is therefore likely that such probe-mapping effects can only be resolved when probes target different regions of the transcript, rather than for probes targeting the same region being affected by biological or technical variability.
