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This study describes a system consisting of a cysteine containing antimicrobial peptide conjugated with silver nanoparticles, in which the two components exhibit a dynamic interaction resulting in a significantly enhanced stability and biological activity compared to that of the individual components.
Based on the use of equivalent concentrations of active Wnt3a, we calculate that the liposomal preparation of Wnt3a exhibits a 5-fold increase in biological activity compared to the isolated protein (Fig. 2A).
Each of the four variants, labeled at the engineered surface cysteines, remain more than 95% monomeric as determined by size-exclusion high performance liquid chromatography (SEC-HPLC) (Figure S2), and retain 80% 130% biological activity compared to wild type [33].
T4 is considered a pro-hormone because of its much lower biological activity compared to T3.
Multivalent conjugates are often touted as having favorable kinetic, thermodynamic, and biological activity compared to that of their monovalent and/or small molecule counterparts.
Human IL-12 secreted by transfected SHSY5Y and IMR-32 cells over a period of at least 10 days in vitro showed a stable level of biological activity compared to control supernatant (P<0.05).
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Most recently, renewed interest has arisen in manufactured silver nanomaterials because of their unusually enhanced physicochemical properties and biological activities compared to the bulk parent materials.
Panduratin A and 4-hydroxypanduratin A were reported to exhibit stronger biological activities compared to other secondary metabolites in Boesenbergia rotunda[ 17].
However, mAb with high mannose glycans had relatively similar biological activities compared to the mammalian-derived mAb overcoming concerns about plant-specific glycoepitopes expressed by others [ 58].
From these data, the AgNP using aloe vera extract (biological synthesis) show a significant activity compared to all the other samples.
As consequence of the increased surface-to-volume ratio, nanoparticles exhibit a potentially higher biological activity compared with larger particles which has been linked to prooxidative but also to antioxidative processes [ 26– 31].
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