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Our method allowed us to leverage data from multiple GWAS loci to identify biologic context for the aggregate findings.
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To identify biologic context for these results, we constructed a coexpression network to define modules of genes with similar patterns of CD4+ gene expression (coexpression modules) that could serve as constructs of broader gene expression.
To explore the biologic context for our GWAS results, we analyzed our GWAS and gene expression findings together.
Our integrated approach can be applied to provide biologic context for GWAS of other diseases.
We adopted such a strategy and were able to identify novel biologic context for allergic rhinitis.
Further, our methods may be implemented to provide biologic context for GWAS of other diseases.
Our methods can be used to provide a richer biologic context for GWAS findings in other disease areas.
Pathways representing specific biologic mechanisms may be overrepresented in genes identified by GWAS, thereby providing relevant biologic context for GWAS results.
Various methods have been tried to identify biologic context for loci identified by GWAS, including (1) expression quantitative trait loci (eQTL) mapping and expression single nucleotide polymorphism (eSNP) analysis [ 10, 15- 18], (2) network analysis [ 19, 20], and (3) pathway analysis [ 18, 21- 23].
The Museum of Paleontology has always recognized the value of fieldwork, both for understanding the geologic and biologic context of fossils and for building its collections.
Finally, detailing of the larger in vivo context for the actions of intranuclear ligand/receptor complexes at the tissue and organismal level will provide a final level of proof necessary to establish the biologic relevance of this process.
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CEO of Professional Science Editing for Scientists @ prosciediting.com