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The biogas content was analyzed using a gas chromatograph equipped with a thermal conductivity detector (GC-TCD), while bio-fuel was analyzed using High Performance Liquid Chromatography (HPLC).
After anaerobic digestion with thermochemical pretreatment, the total chemical oxygen demand (tCOD) reduction, volatile solid (VS) reduction, methane yield and methane biogas content were 88.9%, 77.5%, 0.52 m3/kg VS and 79.5%, respectively.
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The upswing of pH from 5.0 to 5.6 had a significantly more impact on biogas H2 content than the downswing of pH from 5.0 to 4.3.
Figure 1 Epifluorescence micrograph showing cells in the biogas reactor content.
In some cases, part of the biogas reactor content was pumped out to allow the agitators to stir the foam.
As the foaming problem was very serious, the biogas digester content had to be pumped out and the AD process had to be restarted.
The biogas CH4 content from maize silage alone was 50%to52%2%, as found previously [ 47].
The UASB reactor was operated with an initial organic loading rate of 0.42 kg COD/m-day, which was increased to reach 5.6 kg COD/m-day, and obtained COD removal of 93.8% and a biogas methane content of 60%.
The biogas hydrogen content was determined by gas chromatography GC-20100, Shimadzu, Japan) using a thermal conductivity detector (TCD) with argon as the carrier gas, and the column was packed with Supelco Carboxen 1010 Plot (30 m × 0.53 mm i.d).
The optimum loading rate selected in this study was 17.05 kg COD/m-day, which corresponded to the highest biogas methane content of 84%, methane yield of 0.263 m CH4/ kg CODadded and 69% COD removal (the level of COD removal increased in this stage).
These authors used an initial organic loading rate of 0.5 kg COD/m-day, which was increased to reach 5 kg COD/m-day, and obtained removal of 98% of the COD and a biogas methane content of between 70 and 74%.
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