As such, the results from this study could be applied to different biofuel pathways to improve greenhouse gas performance.
Advancements in biorefining technologies along with the development of novel fermentation strategies and analysis will be paramount in order to establish supplementary and sustainable biofuel pathways.
Saccharomyces cerevisiae remains the premier host for metabolic engineering of biofuel pathways, due to its many genetic, systems and synthetic biology tools.
In recent years, the advancement of tolerance engineering and evolutionary engineering provides powerful platform for obtaining host strains with desired tolerance for further metabolic engineering of biofuel pathways.
White dots indicate the default values for the biofuel pathways included in Annex V of the EU Directive 2009/28/EC [7]—rape seed biodiesel for biodiesel (different oil crops), rapeseed HVO for HVO/HEFA (different oil crops).
Novel biofuel pathways with increased product yields are evaluated against conventional lignocellulosic biofuel production processes: methanol or methane production via gasification and ethanol production via steam-explosion pre-treatment.
The ABPP capability may also facilitate the discovery of new biofuel pathways by confirming and discovering putative enzymes and their roles in metabolism and regulation.
While engineering of new biofuels pathways into microbial hosts has received considerable attention, innovations in bioprocessing are required for commercialization of both conventional and next-generation fuels.
Process models within the system boundary were combined to generate an engineering system model that was further leveraged to perform a techno-economic analysis to determine commercial feasibility of the biofuel production pathway.
