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In previous studies, tramadol produced mild euphoria [ 18], but this was not deemed an undesirable attribute of the drug, seem that no other adverse effects regard to platelet aggregation, vomiting, gastrointestinal function, or biochemical values were found in other studies [ 21, 24].
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No missing values were found.
No extreme values were found.
Parameter values are found in Table 1.
Out of range hematological and biochemical values were observed in 21 measurements.
Biochemical values were shown as mean ± SEM and compared and analysed using Students t-tests.
Demographics, clinical, hemodynamic and biochemical values were collected.
These biochemical values were within the normal ranges described in the Mouse Phenome Database [ 28].
All remaining serum biochemical values were within the reference intervals provided by the laboratory.
All biochemical values were measured by an Olympus AU640 autoanalyzer (Olympus, Kobe, Japan) using standard methods.
Notably, several biochemical similarities were found between the HDAC and the AChE.
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