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Here, a method is proposed for estimating the TTN of a given biocatalyst from readily measured biochemical quantities, namely the specific activity and the deactivation half-life, measured under identical conditions.
ii) Rewrote the abstract so that it is now clear that "amplification" refers to the derived relationship between the relevant biochemical quantities, rather than to a literal biological mechanism.
The model shows that the relationship between the relevant biochemical quantities can be viewed as strong linear amplification, where this effect is a consequence of fast non-specific degradation of pre-crRNA.
Authors' response We appreciate the comment that our statements - which were referring to relationships between biochemical quantities - may be confused for literal biological mechanisms, especially if a reader is exposed only to the title/abstract.
Similar(56)
This technique showed superior defect filling, O'Driscoll histologic scoring and biochemical GAG quantity in comparison to microfracture.
It is demonstrated that the nesting of scales described in these relationships implies limits to the retrieval of absolute concentrations of any biochemical constituents or absolute quantities of the amount of scattering material from hyperspectral observations of total scattering.
Further applications of presented method to detecting a variety of chemical or biochemical molecules in low quantities are expected.
Here, new expression vectors are presented that have been designed for the production of proteins in large quantities for biochemical and structural studies.
Membrane proteins are extremely challenging to produce in sufficient quantities for biochemical and structural analysis and there is a growing demand for solutions to this problem.
When more appropriate culture conditions were applied, after initial 16S rRNA sequencing identified the organism in the sample, we obtained florid culture growth, sufficient numbers of bacteria for optimal biochemical testing, and sufficient quantities of DNA for successful MLST analysis of the C.jejuni isolate.
Taking into account the advantages of lepidopteran insect cell culture systems, the expression system described here could prove useful for obtaining sufficient quantities for biochemical and structural characterization of mosquito ORs as well as ORs derived from other insect taxa and exploration of their functional properties.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com