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Use of this molecular chronometer revealed the inability of traditional phenetic (morphological and biochemical) methodologies to accurately reconstruct the natural genetic history of the microbial world.
Based on modern molecular, genetic and biochemical methodologies it is on the one hand possible to identify for example disease-related point mutations and single nucleotide polymorphisms.
In that fateful summer of 1992, Prasher moved from WHOI to the U.S. Department of Agriculture's Animal and Plant Health Inspection Service APHISS) lab, on Cape Cod in Massachusetts, where he worked on identifying pests "using molecular and biochemical methodologies," he says.
Finally, infectious prions can range down in size to oligomers of a few dozen prion protein molecules [18], which would be undetectable by existing biochemical methodologies including MS methods employed in this study.
We investigated this issue more precisely using different genetic and biochemical methodologies.
This has included extending and expanding existing reproductive, behavioral, and biochemical methodologies and end points and examining new ones.
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A finding supported by biochemical methodology and the dual expression of LRP1 and P-EphA2S897 in primary and recurrent GBM tumor specimens.
These results validate the biochemical methodology used in this study.
HbA1c was measured by affinity chromatography (Biochemical Methodology, Drower 4350; Isolab, Akron, OH).
On-line screening systems integrate separation sciences, mass spectrometry, and biochemical methodology, enabling screening for active compounds in complex mixtures.
Identification of the species level was performed by each laboratory of the participant Hospital with either conventional biochemical methodology or an automated system (Vitek 2, bioMérieux, France).
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