Limiting DOT1L inhibitor discovery and ligand optimization have been complex biochemical methods often using radionucleotides and cellular methods requiring prolonged culture.
To better understand the functional implications of these mutations, a variety of biochemical and cellular assays, as well as computational methods, have been developed to assess the impact of the individual mutations.
It involves the use of enormous amounts of data on relevant biochemical and cellular processes, which are generated by "high-throughput screening" methods, and then are combined with substance-specific kinetic data, experimental apical test outcomes and modelling.
Additional details of biochemical and cellular reagents, primers, and protocols are described in Supplementary Materials and Methods.
Metabolism involves a vastly complicated network of biochemical and cellular processes that are linked and that succeed in keeping us alive for as long as they do, but they have these side effects.
However, the underlying biochemical and cellular mechanisms remain unclear.
It rapidly evolves into secondary biochemical and cellular changes (Ghajar, 2000).
Lead compounds exhibited excellent potencies in both biochemical and cellular assays in MYC-dependent cell lines.
The structural data was also used to design inhibitors with improved RORc biochemical and cellular activities.
Using molecular, biochemical and cellular techniques, we probed the cellular functions of ACTN4 in fibroblasts.
