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We have designed a highly reproducible bioassay system to examine this hypothesis.
In the present study we used a bioassay system for measuring plasma cholecystokinin (CCK) to evaluate whether CCK has a physiologic role in regulating gastric emptying in humans.
The bioassay system was fabricated by immobilizing aptamer onto the surface of MNPs, which were employed to capture and concentrate CAP.
With advances in mRNA profiling and detection techniques, we have established a novel and non-radioactive bioassay system using branched DNA (bDNA) technology for detecting protein-therapeutic neutralizing antibodies in patient serum.
The P2X agonist α,β-methylene ATP (10 nmM0.1 mM) also produced concentration-dependent relaxation response that was inhibited by PPADS, SQ-22536 and KT-5720 in the coaxial bioassay system.
In conclusion, the urinary bladder-derived relaxant factor that was recognized by the coaxial bioassay system is neither a peptide of the bladder neurons nor a purinergic mediator but adenylate cyclase and protein kinase A are involved in its release and/or relaxant effect.
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Commercial kits were used to measure urea (Bioassay Systems, Hayward, CA).
* Bioassay Systems Corp., Woburn, Mass., a toxicology testing service, says Timothy W. Devitt has resigned as chairman, president and chief executive officer.
Initial SAR studies, using a variety of bioassay systems were of necessity fragmentary and empirical.
The amount of calcium in the supernatant was measured by colorimetric analysis using a Calcium Assay Kit (QuantiChrom™ Calcium Assay Kit, BioAssay Systems) according to the manufacturer's instructions.
The ATP activity assay was carried out using the QuantiChromTM ATPase/GTPase Assay Kit (BioAssay Systems).
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