In recent years, several synthetic routes have been developed to obtain bioactive molecules derived from lapachones.
Multiple cytochrome P450s, including CYP1A but also CYP2, CYP3, and CYP4 family enzymes, participate in a number of pathways that produce and process bioactive molecules derived from fatty acids, e.g. eicosanoids.
Retinoic acid (RA) is a bioactive molecule derived from dietary vitamin A, which plays an essential role in many basic biological processes such as cell proliferation, differentiation and apoptosis [1].
Phytochemicals are bioactive, non-nutritional molecules derived from plant sources and whose consumption is generally associated with a reduced risk for various diseases, such as cardiovascular disease and cancer [40], [41].
This high-throughput method was utilized to screen a library of 3,228 compounds represented by 1,948 bioactive compounds and 1,280 small molecules derived via diversity-oriented synthesis.
In consonance with previous studies, relying upon the use of antibody or synthetic peptide based cancer treatments for targeting EDA11,13,14, we used an alternate approach based on bioactive compounds derived from natural sources to target EDA.
A variety of commonly used ethno-medicinal plants from the western Himalayan region were nominated and the major 120 lead bioactive compounds derived from them were used for generation of native natural product library.
The effect of ceramide production was then mimicked by treating EpoCD34/HSPCs with synthetic C2- and C6-ceramide, bacterial SMase (bSMase), and the bioactive sphingolipids derived from ceramide C2-dihydroceramide (dihydroceramide) and S1P.
