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Total and individual bioactive compounds were detected by spectrophotometric and HPLC-DAD techniques.
In the literature, seamounts such as the Norfolk Ridge, are regarded as deep sea "islands" with unique biodiversity and restricted species ranges [31], and new bioactive compounds were detected from Norfolk Ridge Ircinia [32].
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Separation was achieved by using a Zorbax SB-C18 column (0.5 × 150 mm, 5 μm; part no: 5064-8256; Agilent, Vivantis company, USA) and the identification of bioactive compounds was detected using Agilent 6520 Accurate-Mass quadruple time-of-flight (Q-TOF) MS system via dual electrospray ionization.
Phenolic compounds were detected in higher amounts than other bioactive compounds.
Similarly various bioactive compounds were isolated from A. javanica[17].
Two bioactive compounds were isolated from C. transvaalensis.
Higher contents of bioactive compounds were found in Lentinula edodes.
All the above estimations of bioactive compounds were carried out in triplicate and means were plotted.
Bioactive compounds were isolated using various chromatographic techniques (VLC, column chromatography, Prep-TLC, etc).
By combining SIM analysis of the products and full-spectrum analysis, unknown inhibitors can be detected and characterized, using the extracted-ion chromatogram (EIC), as shown in Fig. 5, where a fungal extract containing bioactive compounds is profiled.
Bioactive compounds are almost always toxic at higher dose.
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