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In addition, the PINP result strongly indicates that the bioactive agents need to be present in the medium to promote osteogenesis of hMSCS when cultured on 2D substrates.
Bioactive agents such as proteins or nucleic acids are usually too polar to enter cells directly and hence require carriers for cellular delivery44.
From the ctrl samples, it was also demonstrated that, when the bioactive agents were present, a higher cell seeding density resulted in increased ALP activity.
Furthermore, biomaterial injection provides an opportunity to concurrently introduce cellular components and depots of bioactive agents.
Together, these results have implications for the controlled delivery of bioactive agents from silk-elastinlike hydrogels.
Silk-elastinlike protein polymers (SELPs) have been fabricated as matrices for controlled delivery of bioactive agents.
Peptides from natural sources are good starting material to design bioactive agents with desired therapeutic property.
The other is to coordinate the interplays between cells, bioactive agents and extracellular matrix in a natural way.
This is a tissue engineering approach that promises functional restoration of cartilage defects using scaffolds, cells, and bioactive agents.
Carob pulp kibbles, a by-product of carob been gum production, was studied as a source of bioactive agents.
Nitrogen-containing heterocycles are of particular interest and significant importance for the discovery of potent bioactive agents in pharmaceutical industry.
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