AFM images and force-distance curves were recorded using an MFP3D-BIO instrument (Asylum Research Technology, Atomic Force F&E GmbH, Mannheim, Germany).
Levels of interleukin (IL -6 and tumor necrosIL -6ctor (TNF)-α in serum were meandred using tumorio-Plex Reagenecrosisn a Bio-Plex instrument (Bio-Rad Laboratories, Hercules, CA, USA) according to the manufactorer's insTNF -αons.
Antibody measurements were acquired on a Bio-Plex instrument (Bio-Rad), output as mean fluorescent intensity (MFI).
Recently, there has been high demand on miniaturizations of bio-instruments and wide range gas flux measurement in the field of chemistry and mechanics.
The adsorption experiments were carried out in 50-mL conical flasks in a temperature-controlled orbital shaker (QHZ-98A, Taicang Bio-Instrument Manufacture Co., Ltd).
The fluorescence microscope, stereoscopic orientation device, and real-time polymerase chain reaction (PCR) device were supplied by Olympus, Huaibei Zhenghua Bio-instruments Co. Ltd., and iCyderiQ Company, respectively.
Cytokines and adhesion molecules were analyzed using a Procarta Multiplex Cytokine Assay kit from Affymetrix (Freemont, CA, USA) and a Bio-Plex instrument with Bio-Plex software (Bio-Rad, Mississauga, ON, Canada) according to the manufacturers' instructions.
Pooled zebrafish total RNA samples (see above) were processed following the Affymetrix QuantiGene Plex 2.0 Assay Manual using a Bio-Rad Bio-Plex 200 instrument (Bio-Rad, Hercules, CA).
Finally, the absorbance of the prepared solutions was measured at 570 nm on a microplate spectrophotometer (Bio Tek Instrument Inc., USA).
