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Importantly, ADP binds with low affinity, which is insufficient to interfere with binding or release of these high affinity inhibitors.
The in-silico method has enabled us to reveal that a multi-targeted small molecule binds with low affinity to its respective targets.
It is therefore likely that TGFBR1 also binds with low affinity to SB-202190 but was not detected at the threshold of 10 µM used in this study.
In this study, we compared the immunogenicity of a 3D7 VArecombinant6 recombinant protein that binds with low affinity to CSA to a 3D7 VAR2CSA full-length DBL1-6 recombinant protein that binds with nanomolar affinity to CSA [38].
Under the TF independent mechanism, rFVIIa binds with low affinity to the surface of activated platelets.
Several further lines of evidence support the notion that Gβγ binds with low affinity to the GIRK channel.
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As a result of the limited number of residues contacting their binding partner, SLiMs bind with low affinity [usually between 1.0 and 150 micromolar (Diella et al., 2008)] distinguishing them from domain domain interactions that often have an affinity in the nanomolar range (Neduva et al., 2005).
Heparin-binding studies showed that all fragments bound with low affinity to heparin.
In some cases, including those target regions with NL Q<100 NtcA could bind with low affinity, so that relevant binding would require a higher concentration of NtcA, an additional factor, or both (see Figure 3).
Instead, there are many B cells that bind with low affinity to these determinants, a binding which is insufficient for their deletion or inactivation.
The applied growth conditions in complex rich medium might not be suitable for the detection of all PdhR-DNA interactions, although the overproduction of PdhRhis should facilitate binding, also to DNA fragments which might be bound with low affinity.
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