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When the divalent antibiotic coumermycin is added to cells, it binds to this fusion protein and causes it to dimerize.
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Soluble TNFα binds to the fusion protein, becoming unable to act on its cellular receptor.
2 This construct prevents Cre from entering the nucleus and driving recombination until exogenously administered tamoxifen binds to the fusion protein (Fig. 1B).
Lysis and washing buffers contain ATP for removal of chaperone contaminations bound to the fusion protein.
Consistent with this, anti-ΔP avidly binds to a fusion protein containing the carboxyterminal peptide of dynamin 1 (Figure S4B).
These 680 mutations may also play a role in mediating sensitivity to T20, which binds to a fusion intermediate in gp41.
Therefore, in the present study, we designed and examined PI polyamide, which binds to break fusion sites, to inhibit double-stranded breaks.
Motavizumab, binds to RSV fusion protein 70-fold better than palivizumab, and has a 20-fold improvement in neutralization of RSV in vitro.
Furthermore, while the inclusion of the second Ig-like domain of MALT1 in M7A8 somewhat increased its capacity to bind BCL10, this fusion variant could only modestly induce BCL10 ubiquitination and degradation, again suggesting conformational constraints.
Interestingly the non-neutralizing antibodies bound gp41 in the context of the recombinant trimer, whereas the most effective neutralizing mAbs bound to gp41 fusion intermediates.
The proteins bound to GST fusion proteins were visualized by western blotting using antibodies against Nrf2, MafK or JDP2.
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