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This RNA can be visualized by expressing the YFP-MS2 binding protein, which binds to the stem loop structure of the MS2 translational operator as a dimer (Fig. 1A). 3 hours after activation, YFP-MS2 accumulates at the transcription site in 88.3±1.8% of cells (Fig. 1B, panel k; Fig. 1C; Table S1).
These proteins include the antigen receptors of lymphocytes, the (MHC) proteins, the coreceptors involved in cell-to-cell recognition in immune reactions (such as the receptors named CD4, CD8, and CD28), and the FC receptor that binds to the stem of the Y-shaped immunoglobulin molecule.
Specifically, we have shown that siRNA mediated inactivation of MBNL1, which binds to the stem of the CUG hairpin, results in increased dispersion of nuclear foci in DM1 cells [46].
This cytokine receptor is expressed on the surface of hematopoietic cells and binds to the stem cell factor (also known as c-Kit ligand).
Evidence from NMR study has shown that it binds to the stem loop region in H7 HA and stabilizes the neutral pH conformation.
The results are consistent with our idea that the peptide preceding the DUF1115 domain binds to the stem II portion of U4/U6 di-snRNAs.
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(6) Now, we know that hnRNP H binds to the stem-loop IDX ras-ISS1 (Figure 2), but so far our in vitro splicing studies have not thrown light on its action as regards IDX inclusion.
Because of the symmetry properties of this structure, the microprocessor appears to recognize the terminal loop as ssRNA and binds to the stem-loop in an opposite orientation.
MBNL1 binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during the spliceosome assembly, where it regulates the exon skipping of exon 5 through competition with the splicing factor U2AF65.
Although in our study, the selected peptide was unable to bind to the stem cell line of the other species, further proves its specificity, we still observed that several peptides with "homologous core sequence", namely the PWX (L/V) motif, were able to bind to differentiated ES cells, albeit with low efficiency.
One reason for the inverse relationship between morphology and form-meaning compositionality is that inflections such as affixes are, by definition, phonologically bound to the stem, which increases opportunities for phonological compression and sound change to disrupt regular mappings between form and meaning.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com