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In addition, monomethyl arsenous compounds produced when arsenic covalently binds to the reactive thiols of endothelial NO synthetase (NOS) result in inactivation of enzyme activity [ 39].
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The results presented here confirm the successful production of a recombinant single-chain antibody fragment (scFv) retaining the capacity to bind to the SC142-reactive antigen.
The FLICA inhibitor covalently binds to a reactive cysteine residue on the large subunit of active caspase-8 heterodimer.
The hydroquinone was found to bind to the kinase, while the reactive quinoneimine was addressed neither in this publication nor elsewhere in literature.
In order to significantly increase the hydrolysis reaction rate, the controlled respective positioning of the oxime and of the substrate will be achieved through the immunization, and in a second step, the oxime will be bound to the mAb in a reactive position towards the reactive thiophosphate functionality of the substrate.
N-[C-methyl]-l-deuterodeprenyl (C-DED) has been shown to irreversibly bind to the enzyme monoaminooxidase B expressed in reactive astrocytes.
Nearly all of the thiol-reactive proteins bound to the thiol-Sepharose column (as judged by the decrease in MPB labeling), whereas there was little or no binding of thiol-reactive proteins to the control Sepharose column.
This observation reveals that the fluorescein molecule is covalently bound to the Cys residue through the thiol-reactive maleimide linker.
The cross-reactive monoclonal antibodies bind to the region of myosin-II recognized by the largest family of myosin-II monoclonal antibodies.
In paroxysmal cold hemoglobinuria (PCH), polyclonal cold-reactive IgG-antibodies bind to the RBC surface protein antigen termed P but do not agglutinate the erythrocytes.
Based on these results, we have explored the capacity of several bifunctional cysteine reactive small molecules to bind to the hypervariable domain of H-Ras proteins.
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