Paclitaxel binds to the beta-subunit of polymerized tubulin and inhibits the dissociation rate of the tubulin subunits from the tubule.
Moreover, toxin-ADP-ribosylated actin acted like a capping protein, which binds to the barbed (fast polymerizing) ends of actin filaments thereby inhibiting polymerization of non-modified actin and interfering with gelsolin (an 82-kD lipid-binding actin regulatory protein) functions.
For E. coli, extensive studies have shown that membrane binding is mediated by MinD, which polymerizes and binds to the membrane in an ATP regulated fashion [6].
Phenolics in soils can exist as (1) a dissolved form, which moves freely in the soil solution, (2) a sorbed form, which reversibly binds to the soil particle or proteins, and (3) a polymerized form, consisting of humic substances.
TgICMAP1 binds directly to the lattice of bare MTs polymerized in vitro, yet in T. gondii it preferentially binds to the intra-conoid MTs but not other tubulin containing structures, such as the conoid and the cortical MTs.
Purified recombinant eGFP-TgICMAP1 protein binds to in vitro polymerized MTs, suggesting that its interaction with the MT is direct.
Specifically, cyclosporine binds to the cyclophillin receptor.
(C ) Lifetime plots of single cortactin molecules bound to the sides of polymerizing actin filaments, with data collected at two different laser intensities (measured at laser head).
Furthermore, double staining using the S100A14 antibody and phalloidin indicated that S100A14 may bind to membrane-localized polymerized actin, such as cortical actin.
