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The tetR gene encodes TetR which binds to the operators, acts as a repressor and inhibits the expression of both genes in the absence of Tc.
We decrease the rate that TetR2 binds to the operators when it is either free (k3, k5, k64, k65) or bound on Tc (k19, k25, k66, k67), causing a decrease to the binding strength.
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The antitoxin binding inhibits the activity of the toxin, and the stable TA complex binds to the operator of the corresponding TAS operon via the DNA-binding domain of the antitoxin and (auto represses its transcription.
One common method of regulation is carried out by a regulator protein that binds to the operator region, which is another short segment of DNA found between the promoter and the structural genes.
In E. coli, LacI binds to the operator sequence present in the lac operon [25].
LacI binds to the operator region of the lac operon and prevents the transcription of lacZYA genes unless lactose is present in the environment [13].
When lactose is absent from the cell, the lac repressor binds to the operator region of the operon, and blocks RNA polymerase.
A transcriptional inert LexA fusion that binds to the operator located between the UASG and the TATA box is able to repress or block the LacZ expression, a clear indication that the LexA fusion is properly located to the yeast nucleus.
Subsequently, Tc binds to the repressor (TetR2 bound to the operators or free) with high affinity.
As the composition of the operator motif is tightly related to the affinity of the TF for the DNA this finding implicates that some of the LacI-family TFs could potentially bind to the operators of another.
However, when the activated form of repressor which is induced by the attachment of two tryptophan molecules become abundant, it will bind to the operator site and block RNA polymerase from binding to the promoter, thereby, repressing transcription and forming the first feedback loop.
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