IST1 bound with similar efficiencies to full-length ULK3 (residues 1 472) and to a minimal construct that spanned just the C-terminal tandem MIT domains (residues 277 449, hereafter denoted ULK3 MIT 2; Figure 1D, lanes 2 and 5, respectively), but did not bind to the isolated ULK3 kinase domain (residues 1 270; lane 3).
In the stratified epithelium IgM binds to the young (parabasal) cells (IgM1), isolated mature and all aging (top of interstitial layer) cells (IgM2), and a layer of the most superficial cells (IgM3) [ 3, 51] - see also above.
Although GST nephrinICD2 can bind with the isolated first or third PDZ domains of PAR3, all three PDZ domains are required for efficient binding.
The previously identified ATP and CoA aptamers recognize ATP in solution with similar affinities, but neither binds to the affinity resin used to isolate the other [ 8].
The final death toll is bound to rise.Cruelly, the isolated villages were the worst hit.
All phages displaying SBP are bound to and isolated by the magnetic beads.
(A) The first panel shows the P-labeled RNA bound to SAFB1 isolated with or without UV crosslinking separated on a SDS-PAGE gel.
Compared to the crystal structure of the isolated clamp bound to DNA (Georgescu et al., 2008a) the clamp is rotated by ∼20°, resulting in an almost perpendicular orientation (∼80°) with respect to the DNA.
CTB bound strongly to the fraction isolated from untreated Jurkat cells, and also more weakly to the fraction isolated from Jurkat cells cultured with NB-DGJ.
In the hydrophobic fraction, fucose-specific (UEA-I, AOL, AAL and TJA-II) and sialic acid-specific (SNA, SSA and TJA-I) lectins bound preferentially to the proteins isolated from the hPSCs.
