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We show that BLA preferentially binds to negatively charged membranes at acidic pH with higher binding affinity.
The peptide also binds to negatively charged POPC/POPG (3:1) lipid vesicles but fails to insert deep into the membrane interior.
TBO is a cationic dye that binds to negatively charged groups in cells and gives two main spectra of reaction, i.e. pinkish purple for carboxylated polysaccharides such as pectin and greenish blue for aromatic substances such as lignin (Pradhan Mitra and Loqué 2014).
The anticoagulant Annexin V preferentially binds to negatively charged phospholipids such as phosphatidylserine [ 12].
To identify how the Imp2 F-BAR binds to negatively charged phospholipids, the electrostatic surface of the dimer was examined.
The buffer used in the RPLC included tetrabutylammonium hydroxide, which binds to negatively charged entities, making them hydrophobic.
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Secondly, consistent with its function as a DNA binding protein, extracelullar HMGB1 has a natural propensity to bind to negatively charged molecules such as DNA, RNA, nucleosomes and pathogen associated molecules like LPS (an endotoxin) or immune activating cytokines like interleukin-1 (IL-1) (Bianchi, 2009).
Our results show that BLA exhibits a molten globule conformation when bound to negatively charged membranes.
Despite high sequence diversity between the different genera, most parvoviruses bind to negatively charged glycans, such as sialic acid and heparan sulfate, abundant on cell surface membranes.
Chitosan could then bind to negatively charged bacterial surface, facilitating its antibacterial activity.
A positive surface charge is also required for the complex to be able to bind to negatively charged cell surfaces and facilitate cellular uptake.
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