One of these, MIRA-1, had promising properties in vitro but is toxic in vivo, probably because it binds to multiple protein targets extracellularly, resulting in toxicity.
Exon1 at the 5' end and a domain in exon6 near the 3' end, which contain domains that bind to multiple proteins, have evolved faster in primates than in other mammals.
The background of this statement lies in the fact that most drugs are able to bind to multiple protein targets in the human body, this is known as polypharmacology.
Vinculin binds to multiple focal adhesion and cytoskeletal proteins and has been implicated in transmitting mechanical forces between the actin cytoskeleton and integrins or cadherins.
The viral regulatory protein binds to multiple copies of the ACCN6GGT motif that occur in the long control region (LCR) of all HPVs [ 10, 11].
Panomics´ SH3 domain Arrays are designed to determine whether a protein of interest binds to multiple SH3 domains (Panomics).
The results from the protein microarray analysis indicated that phosphorylated Y992 (pY992) bound to multiple cellular proteins, serving as a multifunctional docking site of EGFR [38].
As exemplified by HIV capsid, small molecule inhibitors can bind to multiple binding sites on the capsid protein and induce or prevent protein interactions and conformational changes.
The MSL proteins bind to multiple loci within the X inactivation center (XIC).
Several groups have tested these overall ideas further via bioinformatics studies on collections of hub proteins, and these studies support the common use of disordered regions by hub proteins to bind to multiple partners [ 130- 134].
