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Consistent with this, anti-ΔP avidly binds to a fusion protein containing the carboxyterminal peptide of dynamin 1 (Figure S4B).
These 680 mutations may also play a role in mediating sensitivity to T20, which binds to a fusion intermediate in gp41.
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This notion is supported by findings reported by Tian and colleagues [ 82], who showed that although HMGB1 binds to a RAGE-like man-made fusion protein (RAGE-Fc), binding is much better when HMGB1 is complexed with CpG-rich oligodeoxynucleotides.
This optimized fusion protein toxin binds to a high- or intermediate-affinity receptor.
A total of 250 scFvs were identified that bound to TRAIL-R1, but did not bind to an irrelevant fusion protein.
Motavizumab, binds to RSV fusion protein 70-fold better than palivizumab, and has a 20-fold improvement in neutralization of RSV in vitro.
Soluble TNFα binds to the fusion protein, becoming unable to act on its cellular receptor.
When the divalent antibiotic coumermycin is added to cells, it binds to this fusion protein and causes it to dimerize.
Therefore, in the present study, we designed and examined PI polyamide, which binds to break fusion sites, to inhibit double-stranded breaks.
The exocytosis of secretory proteins may be regulated, whereby a ligand must bind to a receptor to trigger vesicle fusion and protein secretion.
The fusion protein bound to a Protino-Ni column (Macherey-nagel) was eluted with buffer A containing 250 mM imidazole after the column had been washed with buffer A. The eluted fraction was dialyzed against buffer containing 0.05 M Tris-HCl (pH 8.8), 1 mM EDTA and 10% glycerol.
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