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This finding is relevant to our results in that the antibody to H3K27me3 used for these experiments, while highly specific for methylated H3K27 in peptide binding assays, binds only weakly to peptides that are also phosphorylated at K28 [ 79].
However, contrary to some hydroxylated metabolites of other commonly measured environmental chemicals, such as PCBs and PBDEs, BPA binds only weakly to the transport proteins transthyretin and thyroxine-binding globulin (Marchesini et al. 2008).
If 80% of the apo DHFR has an unfolded A−B loop and binds only weakly to ligands, this would effectively reduce the ligand binding constant to apo DHFR by a factor of 5 compared to fully folded DHFR.
The signal was barely above the noise level, thus suggesting either that NH4+ binds only weakly to nucleotide-free DnaK-ABD, or that the binding sites are solvent-exposed and do not shield the ammonium protons from exchange with bulk solvent.
Starches without amylose make brown-red complex, as iodine binds only weakly to the short helical segments in amylopectin molecules.
The novel fusion protein with optimized interdomain linker length will be a useful tool for the purification and detection of various IgGs including mouse IgG1 that binds only weakly to natural protein A.
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In many proteins, SAM and/or PNT domains act as protein protein interaction modules, an important mechanism of biological specificity for ETS factors, which often bind only weakly to DNA in the absence of binding partners or posttranslational modifications [ 3, 12].
The carbon dioxide molecule was predicted to bind only weakly to nanotubes, and the tube-molecule interactions can be identified as physisorption.
Remarkably, XAP2 and PP5, which also reduced PR's transcriptional activity, bound only weakly to the complex.
These findings were confirmed by surface plasmon resonance experiments (Biacore); boophilin showed high affinity for free human α-thrombin, but bound only weakly to PPACK-thrombin or prethrombin-2 (not shown).
TIMP-3 bound strongly to the immobilized PPS, whereas TIMP-3 K26/27/30/76E+R163/K165Q) TIMP-3 K26/27/30/76E+R163/K165Qd PPS.
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