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However, the SNARE helix of VAMP7 binds back onto its longin domain, outcompeting Hrb for binding to the same groove and suggesting that Hrb-mediated endocytosis of VAMP7 occurs only when VAMP7 is incorporated into a cis-SNARE complex.
Using protein crystallography, we show that AP2 undergoes a large conformational change in which C-μ2 relocates to an orthogonal face of the complex, simultaneously unblocking both cargo-binding sites; the previously unstructured μ2 linker becomes helical and binds back onto the complex.
First the actin filament is brought to interact with the HMM-coated surface to form rigor bonds, then it is pulled away at constant velocity in a direction orthogonal to the motor surface (z, parallel to the axis of the trapping laser, velocity 25 nm s−1) to break the rigor bonds one at a time while avoiding that the detached HMM binds back (Fig. 1a).
These studies propose that the "closed" non-phosphorylated cortactin form assumes an autoinhibitory conformation where the SH3 domain binds back to an unidentified site within helical proline-rich (HP) domain to prevent binding to N-WASp and other SH3 ligands.
Hence, tTG is not secreted as soluble protein and then binds back to the cell surface and the ECM as proposed earlier [32], but appears first on the outside leaflet of the plasma membrane and only later is translocated to the ECM.
After reassembly of PSII, PsbO binds back.
Similar(48)
Ignore every rejection and bound back again.
An apparently perfect approach shot from 140 yards hit the pin, causing the ball to bound back down the fairway.
"If it gets unbearable for them, athletic types are going to bound up the stairs, jog down the hall, and bound back down again.
Priced at 35 cents and bound back to back with a reprint of "Narcotic Agent," a memoir by Maurice Helbrant, "Junky" sold 100,000 copies in its first year.
"Practically, the system amounted to a form of remuneration for lost slaves," Mr. Rael wrote, "since freed children were often bound back to their former masters".
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