Antibody clone 6_2G3 derived from a patient with stage III disease (Patient 6, Table 1) was not observed to bind to fibroblasts or melanocytes, but bound to a proportion of melanoma cell lines tested (Figure 5).
They also bound to a proportion of poorly encapsulated C. neoformans cells (which display β-glucan on their surface) and C. albicans yeast cells, although with large cell to cell variations in labelling intensity (Figure 1, Panel A, c d; g h).
Unbound phage could be washed away, leaving bound phage, a proportion of which was specific for the target antigen.
Vitamin D metabolites in the circulation are predominantly bound to DBP; however, a proportion of an oral dose, particularly for the less-polar vitamin D, will initially be associated with chylomicrons that may further influence initial metabolism and sequestration into body tissues.
Thus, the complement-coated IgA1 antibodies retained their antigen-binding function, especially as a proportion of their covalently bound C3b progressively degraded to iC3b and C3d.
Genome-wide studies of CREB in hepatocytes have not examined CREB DNA binding dynamics, but have shown that CREB binds a large proportion of gene promoters that are not induced by cAMP [ 6, 14].
For instance, TET1 binds a significant proportion of Polycomb and Trithorax group target genes.
Cells lacking MUS81 or EME1 show defects in the repair of DNA interstrand crosslinks (ICL) resulting in hypersensitivity to agents such as mitomycin C. In metazoans, a proportion of cellular MUS81-EME1 binds the SLX4 scaffold protein, which is itself instrumental for ICL repair.
Alternatively, it may simply reflect limited accessibility of the mAb epitope to PTB within a proportion of the membrane bound replication complexes.
The fact that Ozz and its direct partner Elo C could be co-immunoprecipitated from sarcomeric but not soluble myosin extracts of E16.5 embryonic muscle indicates that at this age a proportion of Ozz molecules bound to MyHCemb is assembled into the E3 ligase complex.
