Furthermore, the C terminus of CHMP2B is necessary for binding VPS4 (26, 26); and CHMP2B truncation mutants lose this VPS4 binding domain so would be predicted to fail to dissociate from the endosomal membrane.
Two of the SNPs are within 200 bp of transcription factor binding sites and would be predicted to have an effect on transcriptional regulation (Table 5).
As proof-of-concept, we designed peptides that contain the conserved GWxQ binding motif and would be predicted to compete with the native interaction.
In AgtGS, His163 and Phe167 are involved in glycogen binding [ 43] and would be predicted to be found in the GT-5 domain of SSs.
For the E792, MC1, and MC2 mutations this could be expected, as these are all predicted to perturb the interaction between dCPAP and dSTIL (as is the case with similar mutations in zebrafish CPAP in our in vitro binding assays), and this would be predicted to perturb the recruitment of dCPAP to centrioles.
It is currently unknown if CENP-A is recruited by, or competes for transcription factor binding sites, either of which would be predicted to impact gene expression.
Basically, we feel that the previous studies had technical flaws and, in both cases, binding was done under conditions that would be predicted to favor protein oligomerization which would give an artificially higher binding affinity.
The ability of Wit to inhibit STAT3-receptor binding, tyrosine phosphorylation, and P-STAT3 nuclear accumulation would be predicted to result in blocking STAT3 DNA binding.
The transamidating activity and GTP binding behavior of the constructs was as would be predicted based on our knowledge of the structure and function of TG2 [10] [11], [11].
Succinylated/malonylated K503 would be predicted to prevent binding of the allosteric inhibitor GTP.
