Sentence examples for binding with the membrane from inspiring English sources

To further assure that compounds are binding with the membrane ergosterol, the cells were incubated with compounds for a definite period of time and amount of unbound compound in supernatant was detected spectrophotometricaly.

KAHRP binding with the membrane skeleton leads to an increased rigidity, blockage of blood vessels and resistance to flow (Pei et al., 2005).

The arrangement of these unique densities is such that they are likely involved in binding with the membrane-bound a subunit at the V1VO-interface [19], [26].

During autophagy induction, LC3-I, a cytosolic form of LC3, is converted to LC3-II by lipidation, which facilitates its binding with the membranes of autophagosomes (Kabeya et al, 2000).

The substantial conformational changes in the B′ helix and F G loop regions are induced upon ligand binding, consistent with the membrane nature of the protein and its substrate.

The in vitro experiments showed that the released anti-VEGFR-2 antibody has receptor-specific binding with the HUVEC membrane, which proves that the bioactivity of the protein has been preserved during the encapsulation and release.

With the absence of considerable binding with membrane the nanoparticles can easily diffuse to the cellular interior regions (for detailed theoretical analysis see above [ 46]).

The least amount of binding of compounds with the membrane ergosterol was observed for this strain, whereas the increase in cell density of CA04 resulted in increased binding of both the test compounds with the ergosterol.

To prevent nonspecific binding of antibodies with the membrane, it was treated with 5%% (w/v) solution of dry milk in TBS-T (150 mM NaCI, 20 mM Tris, 0.1 % Tween 20, pH 8.0) buffer.

Highest titers were observed with one particular DARPin binding to the membrane distal domain of HER2/neu with medium affinity.

Before and after application of 25 µl of the binding reaction, the membrane was washed with 180 µl of wash buffer.