Methods: We used X-ray crystallography to obtain atomic detail of the binding modes of TBBPA and 3-OH-BDE-47 to estrogen sulfotransferase for comparison with binding of the endogenous substrate estradiol.
To block the binding of the endogenous Sema3 with Nrps, we used function-blocking antibodies that specifically blocked ligand-binding sites in Nrps.
Among the LTR elements with Pol II binding, the endogenous retrovirus HERV-Fc1 displayed a large-degree of Pol II and Pol II S2 enrichment with the most prominent binding in a K562 CML line.
The binding activity of purified MBP-CCR3 was verified by binding with its endogenous ligands CCL11 and CCL24, with KD of 2.90 × 10−6 and 1.81 × 10−6 M, respectively.
The procedure provided by the mouse-on-mouse peroxidase immunodetection system (M.O.M. Kit; Vector) was used to eliminate any nonspecific binding of anti-mouse secondary antibodies with the endogenous mouse immunoglobulins in the tissue, according to the manufacturer's instructions.
Characterization of site1 (Fig. 7B) shows a large overlap with the endogenous ligand-binding region.
The At80MD truncation protein may be able to compete with the endogenous AtMYB80 for binding the promoters of target genes, but fail to activate gene expression.
In principle, HD should efficiently compete with the endogenous Hbox12 for binding to DNA, quenching the repressor activity on target genes.
Some plants contain distinct families of phytocompounds, which are structurally similar to steroid hormone, 17 β-estradiol (E2) and compete with the endogenous hormone for binding to estrogen receptor (ER), thus reducing the hormonal effect of endogenous estrogens [ 2– 4].
One postulated mechanism for functional selectivity (that may fit well with experimental data on the biological action of aripiprazole) is that the target drug may trigger different conformation changes, compared with the endogenous ligand, when binding to the receptor-G protein complex.
A gel mobility shift assay showed that transfected NRE TFD blocked endogenous NREB binding with the rat renin gene.
