We found that Scr dimers are predominantly formed on the DNA and are equally abundant at the chromosomes and an introduced multimeric fkh250 binding-site, indicating different mobilities, presumably reflecting transient binding with different affinities on the DNA.
Blon_2344 and Blon_2347 specifically recognized type 2 glycans (Figure 1), binding with different affinities linear and branched polylactosamines, as well as lacto-n-neotetraose (LNnT) and difucosyl lacto-N-hexaose.
Whether these are the same with the HLA.A2.1-restricted ones binding with different affinities to the mouse class I alleles, or represent distinct ones, remains to be determined.
TFs bind to a broad spectrum of binding sites with different affinity and change targets widely among species.
The geometry of the A·A pair is different to the G·A pair, creating a binding pocket with different affinity for the ring I of aminoglycosides, more favorable to the interaction of the antibiotic with the prokaryotic ribosome.
Further confirmation that this displacement curve indicates the existence of two separate binding sites with different affinity was obtained in experiments where the stable GppNHp was added in excess to dissociate all receptor G-protein complexes.
CCAAT/enhancer binding proteins (C/EBP) include multiple family members that bind to CEBP binding sites with different affinities; that may influence the precision of the present prediction.
Another important factor that may contribute to the variability of radioligand binding to TSPO is the presence of several binding sites with different affinities dependent on single nucleotide polymorphism of TSPO, at Ala147Thr [26].
The multiple-binding-site hypothesis was tested with the Virtual Cell software, assigning to NS1-deYFP two distinct and immobile binding sites with different affinities.
PRRII sequence contains three AT-tracts, separated by 6 and 7 bp, the longest one being 19 bp long, corresponding to three potential binding sites with different affinities for HMGA1.
