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These data showed that HL binding with a minor groove of DNA was the most stable.
Therefore, C60+HL binding with a minor DNA groove is suggested to be the most probable version of C60 fullerene, HL, and DNA combined interaction.
In the case of C60+HL version, the binding with a minor DNA groove (Fig. 4d) occurred via CGC-GCC nucleotides.
The binding with a minor DNA groove in the case of HL+C60 version occurred via GCTA-GCAT nucleotides (Fig. 4a).
Int values were 8.7 kJ/mol in the case of HL intercalation into DNA, 6.3 kJ/mol in the case of its binding with a major DNA groove, and 3.6 kJ/mol in the case of its binding with a minor DNA groove.
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Metal ion titrations reveal strong Mg2+ binding to a minor groove shelf in the D5 bulge.
Fig. 4 Combined interaction of diphenyl-N- trichloroacetyl -amidophosphate (HL) andiphenyl-N- trichloroacetyl -amidophosphateversions): a, b bindiphenyl-N- trichloroacetyl -amidophosphateercalation into minor andiphenyl-N- trichloroacetyl -amidophosphate
Previously with the use of computer simulation, we have demonstrated that C60 molecule could interact with DNA and form a stable C60+DNA complex when binding with DNA minor groove [15].
Morrow writes: "Muhammad also insisted that the Law of Moses remained binding with some minor modifications which resulted from the final revelation".
The corresponding dye-labeled probes at 200 nM were 6FAM-TTCGGTACTAACTTTTAGTTAA and VIC-CGGTACTAACCTTTCGATTA modified with a minor groove binding (MGB) protein (Applied Biosystems).
We have developed a technique based on double staining of the DNA with a minor groove binding (4", 6-diamidino-2-phenylindole (DAPI)) and a base pair intercalating (propidium iodide (PI) or SYBR green) fluorescent stain and color deconvolution.
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binding with a strong
binding with a major
binding with a hydrophobic
binding with a great
binding with a moderate
binding with a corresponding
binding with a macromolecule
binding with a high
binding with a similar
binding with a fixed
binding with a slow
binding with a further
binding with a vascular
binding with a specific
binding with a genetic
binding with a large
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