In a different situation, transient binding to standalone proteins with a limited choice of affinities is able to induce this kind of slowdown [38], [39].
However, for hESC the flow cytometric analysis is more complicated compared to analysis of other cell lines due to high non-specific binding, autofluorescence and a limited number of antibodies with high specificity.
As shown in Figure 5B and Figure S4, Inhibitor VIII has several hydrophobic contacts with AKT1 that appear to drive compound binding while only a limited number of polar contacts are observed.
We hypothesized that this category of proteins, by bridging highly connected parts of the cellular network with a limited number of binding events, could be critical checkpoints for the control and regulation of the cellular information flow.
Kim and co-workers [ 39] found that disordered regions could function as interfaces with a limited number of binding partners, particularly in the context of phosphorylation cascades in signalling pathways, where proteins tend to contain both a structured kinase domain and an unstructured kinase-binding domain.
In species with dry stigmas, including Arabidopsis thaliana, this interaction is highly selective, allowing binding of only a limited pollen grain set.
This suggests that this element could be the binding site for a limited activating factor.
Consistent with a limited effect on microRNA pathways, reporter vectors containing microRNA binding sites were here observed to be invariably regulated during Salmonella infection.
Consider a realistic query in which a researcher asks for a slightly alkaline compound with several substructures, available results in bioassays with high activity, limited molecular weight, and known binding with a protein.
A high number of different synthetic peptides are competing to interact with a limited amount of HLA molecules, giving a selective force in the binding.
