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The modular architecture of SdaI, wherein one domain mediates DNA binding while the other domain is predicted to catalyze hydrolysis, distinguishes SdaI from previously characterized restriction enzymes interacting with symmetric recognition sequences.
The properties of the mutant proteins suggest that one metal ion plays a role in substrate binding while the other is involved in catalysis of the exonuclease reaction.
Among the nucleic acid binding motifs of helicases (namely motifs Ia, III, IV, V), the motif Ia is devoted to oligonucleotide binding, while the other motifs have also additional and not yet well defined functions [35].
Both the triple mutant and the F296A mutation abolished VAPB binding, while the other two point mutants retained the wild-type ability to interact with VAPB.
The figure shows a 200 Kb region that contains the white gene, half of which exhibits strong DCC binding while the other half does not.
Indeed, FIR is the only example of a multi-RRM protein in which one RRM domain participates in nucleic acid binding while the other RRM domain is sequestered in protein contacts.
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As the optimization problem (P1) is convex with linear constraints, at the optimum point some constraints are binding, while the others are non-binding.
We synthesized a heterobifunctional polymer-peptide system with one end binding HA while the other end binding either sialic acid-containing glycosylated transmembrane molecules on the ocular surface epithelium, or type I collagen molecule within the tissue matrix.
One subunit contains both the substrate and AMP binding pockets while the other subunit covers the opening to the binding sites (Fig. 7A).
One encoded the predicted full length leptin binding domain while the other cDNAs were shorter as a consequence of different deletions in exon 9, and one had a further deletion in exon 10.
Four variants associated with sulfite oxidase deficiency have been identified: two mutations are near the sulfate binding site, while the other mutations occur within the domain mediating dimerization.
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binding while the COOH-terminal
binding while the amino-terminal
binding while the corresponding
binding while the dominant
binding while the concerted
binding while the C-terminal
binding while the positive
binding while the third
binding while the empty
binding while the double
binding while the low
binding while the lysine-substituted
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