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(The corresponding RMSDs between the GBP domains are 0.6 Å and 0.8 Å.) These strong domain-specific similarities indicate that the large and small domains move more or less intact upon ligand binding, which is indicative of a hinge-like motion at the domain boundaries [43].
21– 23 Site-selective fluorescence labeling studies have also shown that the magnitude of fluorescence changes upon agonist binding, which is indicative of a conformational change induced by the agonist, correlates with intrinsic activity.
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Moreover, the values of binding constant were found to be in the range of 103 M− 1, which is indicative of groove binding between ticlopidine and calf thymus DNA.
There is also evidence of receptor binding specific to PACAP in the LC, PAG and also dorsal raphe nuclei, which is indicative of PAC1 binding [95].
Indomethacin but not nabumetone or DAHP added to the sub mitchondrial particles significantly decreased the intensity of the latter featrures (Fig. 1) (g ~ 2.04 and 1.99) suggesting formation of radical species which is indicative of binding of indomethacin to and the inhibition of the respiratory chain direct to this site.
A Hill coefficient value of 2.04 is measured for the control sequence, which is indicative of near-simultaneous binding of the two ATP moieties.
The interaction between the apoenzyme and PLP is characterized by a dissociation constant of 0.09 μM, which is indicative of very tight binding of the cofactor to the enzyme.
Furthermore, people can document in advance specific wishes for end-of-life care in an advance statement, also called a positive AD, which is indicative but not legally binding on the physician.
Binding constant of the amsacrine DNA complex was found to be K = 1.2 ± 0.1 × 104 M−1 which is indicative of moderate type of binding of amsacrine to DNA.
We observed a very steep response over a small concentration range of indole, which is indicative of biological significance for odor binding to an OBP.
Interestingly, the concentration of the luminal Ca2+-binding proteins sarcalumenin and calsequestrin is considerably increased in WR muscle, which is indicative of a greater demand for Ca2+-buffering within the SR.
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