To create an artificial genetic switch that acts at the level of transcription, we fused a small molecule binding aptamer to a previously evolved RNA that activates transcription when localized to a promoter.
In contrast, when localized to the mitochondria, SIRT5 promotes apoptosis (Fig. 1C).
Thus, categorizing p21 and p27 as tumor suppressors when localized to the nucleus, and oncogenic when localized to the cytoplasm, is probably an oversimplification.
Fibronectin binding was localized to IG4-5 and fibulin-2 binds to IG2 and IG13-15 with different affinities.
The SH3 binding sites of two SH3 binding proteins were localized to a nine- or ten-amino acid stretch very rich in proline residues.
The RyR2 binding site was localized to a highly conserved 150-amino acid residue region.
More than half of the EWS/FLI1 binding sites were localized to intergenic regions.
S100B is a calcium-binding protein localized to astroglial cells [ 30].
Candidate Efg1-binding proteins localized to the EFG1 promoter include Czf1, Flo8, Sfl1, Sfl2 and Ndt80 proteins [ 11, 12, 25].
A successful ovarian cancer screening program would enable us to detect the cancer earlier, when it is localized to the ovary and treatment is more effective.
